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Introduction

The ‘macrophage pathway analysis’ suite provides a workflow for

  • curating a signal transduction pathway network describing transmember receptor (TM)-dependent activation of gene regulation
  • performing ‘shortest path’ analyses that link transmembrane receptors to gene up/down regulation

Click Here to access our Colab-based NetworkX Script

[NOTE] To fully utilize the given script, you must follow the instruction first. Please, get familiar with the protocol we provide in this Tutorial.

Required Software

  • Cytoscape: Provide a great graphic support along with the large database of pathways (Signor, Wikipathway, etc.)
  • Colab: Cloud-based iPython Notebook provided by Google
  • Python: For the beginner, Kaggle should be useful and informative. You can register yourself with Google account.

Optional Software

  • PathwayAnalysis: The backend code for the pathway analyses routines called in the colab notebook listed above.

This tutorial will cover …

  • Visualization and curation of a signal transduction network (see Cytoscape)
  • Pathway analyses for a signal transduction network (see Colab)
  • Advanced topics including (see Misc) – Input files and their structure – How to upload files – Calculation details